Evaluating the Effects of Enrofloxacin on Angiogenesis Using the Chick Embryo Chorioallantoic Membrane Model

ObjectiveBecause little information was available in the literature about the angiogenic property of enrofloxacin, this investigation was undertaken to evaluate this aspect using an in vivo model. DesignExperimental study. AnimalsTwenty fertile chicken eggs (Ross 308) with the average egg-weight of 55 ± 0.4 g were randomly divided into experimental and control groups. ProceduresAgar pellets were prepared with enrofloxacin concentrations at a dosage of 10 mg/Kg eggweight. A window was carved on the eggshell and drug-impregnated pellet applied on day 8 of incubation on the surface of the chick chorioallantoic membrane. The response of vascular plexus to the drug application was evaluated. ResultsOur results showed that enrofloxacin decrease angiogenesis as shown by a reduction in the morphometric parameters of vascular plexus including total vessel's length, the number of vascular branch, vascular complexity and capillary density (p<0.05). Conclusion and Clinical RelevanceBased on findings, it is suggested that enrofloxacin offer a new class of anti-angiogenic agents that might augment the drugs available for the clinical treatment of angiogenesis related diseases. KeywordsAngiogenesis, Chorioallantoic membrane, Enrofloxacin.